Microbiological and Physiochemical Analysis of Sachet Water within Awka Metropolis
DOI:
https://doi.org/10.59653/ijmars.v4i01.2119Keywords:
Microbiology, Physicochemical Analysis, Sachet water, AwkaAbstract
Drinking water quality is one of the major significances affecting human health, lifestyle and economic well-being. Drinking water quality is solemnly dependent on the quality of source water, the treatment in water treatment plants before distributed, the water distribution system and the containers/tanks used for water storage and the household filters. This study evaluated the bacteriological, physicochemical, and heavy metal qualities of ten popular brands of sachet water commercially sold in Awka Metropolis, Anambra State, Nigeria. The aim was to assess their compliance with national and international drinking water standards and determine their potability. Standard microbiological techniques were employed to quantify microbial loads, isolate and identify bacteria, while atomic absorption spectrophotometry was used for heavy metal analysis. Physicochemical parameters were determined using standard American Public Health Association (APHA) methods. The results indicated that while the majority of the water brands were free from significant microbial contamination, two brands (FC and DS) tested positive for total coliforms, with brand FC also testing positive for faecal coliforms (E. coli), indicating faecal pollution. Bacterial isolates of public health concern included Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Enterobacter aerogenes, Staphylococcus aureus, Streptococcus faecalis, and Bacillus subtilis. Fungal counts were low but present in some samples. The physicochemical parameters (pH, turbidity, temperature, hardness, colour, and taste) for all samples were within the acceptable limits set by the WHO and NSDWQ. However, heavy metal analysis revealed alarmingly high levels of lead in one brand (FC), which exceeded the WHO permissible limit by over 12 times. The findings reveal that despite generally acceptable physicochemical qualities, some sachet water brands in Awka Metropolis pose potential health risks due to microbiological contamination and toxic heavy metals. The study recommends stricter regulatory monitoring, improved hygiene practices among producers, and public awareness to safeguard consumer health.
Downloads
References
Adam, A. (2014). Consumer confidence in the Nigerian sachet water industry. Unpublished manuscript.
Addo, M. A., Akuoko-Asibey, A., & Addo, I. A. (2016). Quality of sachet water in the Teshie-Nungua area of Accra, Ghana. Journal of Environmental and Public Health, 2016, 1-7.
Adegeko, A. A., Stumble, M. B., & Odeyemi, A. T. (2012). Microbiological quality of sachet water in Abia State, Nigeria. Journal of Environmental Science and Water Resources, 1(2), 1-5.
Adegoke, A. A., Adeleke, O. E., & Adegoke, O. A. (2012). Microbial quality of sachet water in Osogbo, Nigeria. European Journal of Experimental Biology, 2(5), 1607-1611.
Adenkunle, L. V., Okonko, I. O., & Adewoye, S. O. (2004). Bacteriological assessment of some bottled and sachet water in Ibadan, Nigeria. Science Focus, 7, 115-120.
Adetunde, L. A., Glover, R. L. K., & Oguntola, G. O. (2014). Assessment of the quality of sachet water consumed in the Wa Municipality of Ghana. Research Journal of Applied Sciences, Engineering and Technology, 7(11), 2277-2281.
Adetunde, O. T., Ogbole, J. O., & Oluwole, A. O. (2021). Assessment of heavy metals in sachet water in Zaria, Nigeria. Journal of Environmental Chemistry and Ecotoxicology, 13(1), 12-18.
ADWG. (1996). Australian drinking water guidelines. National Health and Medical Research Council.
Afiukwa, F. N., Oko, O. S., & Agah, M. V. (2010). Bacteriological quality assessment of sachet water sold in Abakaliki metropolis, Ebonyi State, Nigeria. Nature and Science, 8(10), 1-5.
Agu, K.C. and Chidozie, C.P. (2021). An Improved Slide Culture Technique for the Microscopic Identification of Fungal Species. International Journal of Trend in Scientific Research and Development, 6 (1): 243-254, URL: www.ijtsrd.com/papers/ijtsrd45058.pdf
Agu, K. C., & Odibo, F. J. C. (2021). Biodegradation potentials of Aspergillus flavipes isolated from Uburu and Okposi Salt Lakes. International Journal of Trend in Scientific Research and Development, 5(5), 1160-1170. http://www.ijtsrd.com/papers/ijtsrd44949.pdf
Agu, K. C., Nmecha, C. O., Nwaiwu, M. O., Ikedinma, J. C., Awah, N. S., Eneite, H. C., Victor-Aduloju, A. T., Umeoduagu, N., & Onwuatuegwu, J. T. C. (2017). Isolation and characterization of halotolerant bacteria from Ezzu River Amansea, Awka, Anambra State. Bioengineering and Bioscience, 5(4), 86-90. https://doi.org/10.13189/bb.2017.050303
Agu, K. C., Orji, M. U., Onuorah, S. C., Egurefa, S. O., Anaukwu, C. G., Okafor, U. C., Awah, N. S., Okafor, O. I., Mbachu, A. E., & Anyaegbunam, B. C. (2014). Influence of solid waste dumps leachate on bacteriological and heavy metals contamination of ground water in Awka. American Journal of Life Science Researches, 2(4), 450-457.
Agu, K. C., Umeoduagu, N. D., Egurefa, S. O., Awari, V. G., Uwanta, L. I., Ikenwa, B. O., Udenweze, E., Nwiyi, I. U., Chidubem-Nwachinemere, N. O., Ozoh, C. N., Ohanazoeze, C. F., & Nwosu, J. C. (2023). Comparative study of the microbiota of fish ponds in Awka, Anambra, Nigeria. Global Scientific Journal, 11(6), 1625-1646. http://www.globalscientificjournal.com/researchpaper/Comparative_Study_of_the_Microbiota_of_Fish_Ponds_in_Awka_Anambra_Nigeria.pdf
Agunwamba, J. C. (2000). Water engineering systems. Immaculate Publications.
Ajayi, A. A., Sridhar, M. K. C., & Adekunle, L. V. (2008). Quality assessment of bottled and sachet water in Ibadan, Nigeria. African Journal of Biomedical Research, 11(2), 251-254.
Ajewole, K. (2005). Water supply and sanitation in Nigeria. Nigerian Environmental Study/Action Team (NEST).
Ajibade, W. A., Adewumi, J. R., & Vanloon, G. W. (2015). Assessment of the water quality of bottled and sachet water in Ibadan, Nigeria. Journal of Applied Sciences and Environmental Management, 19(2), 259-264.
Akinde, S. B., Sunday, J. T., & Obire, O. (2011). Bacteriological quality of packaged drinking water brands marketed in Port Harcourt, Nigeria. Nature and Science, 9(10), 1-5.
Alli, J. A., Okonko, I. O., & Alabi, O. A. (2011). Parasitological evaluation of sachet water samples in Southwestern Nigeria. Nature and Science, 9(10), 1-5.
Amaeshi, L. O. (2016). Evaluation of sachet water production processes in Saki town, Nigeria. Unpublished manuscript.
Amori, A. A., & Makinde, A. A. (2012). Water supply and sanitation in Nigeria: A review. International Journal of Development and Sustainability, 1(3), 949-962.
Ante, I. O., Oko, O. J., & Umeh, S. I. (2007). Microbiological quality of sachet water produced and sold in Kaduna metropolis, Nigeria. Journal of Applied Sciences, 7(11), 1453-1457.
Anyamene, C. O., & Ojiagu, D. K. (2014). Bacteriological quality of sachet water sold in Awka Metropolis, Anambra State, Nigeria. International Journal of Engineering Science Invention, 3(9), 01-05.
APHA. (1998). Standard methods for the examination of water and wastewater (20th ed.). American Public Health Association.
APHA. (2005). Standard methods for the examination of water and wastewater (21st ed.). American Public Health Association.
Ashaye, O. A., Adeyemi, E. A., & Sanni, L. O. (2001). Physicochemical properties of sachet water in Ibadan, Nigeria. Nigerian Food Journal, 19, 45-50.
Aslund, P. (1984). Fungi in drinking water: A case study from Finland. Proceedings of the 4th International Mycological Congress.
Ateba, C. N., & Maribeng, M. D. (2019). Detection of Staphylococcus aureus in potable water and its implications for public health. Water SA, 45(2), 220-228.
Awari, V. G., Umeoduagu, N. D., Agu, K. C., Okonkwo, N. N., Ozuah, C. L., & Victor-Aduloju, A. T. (2023). The ubiquity, importance and harmful effects of microorganisms: An environmental and public health perspective. International Journal of Progressive Research in Engineering Management and Science, 3(12), 1-10.
Ayokunle, O. (2011). Water treatment process in sachet package water factory.
Balogun, K. J., Adekunle, L. V., & Oloruntoba, E. O. (2014). Bacteriological quality of sachet water sold in parts of Lagos State, Nigeria. African Journal of Microbiology Research, 8(20), 2048-2052.
Banu, R. A., & Menakuru, H. (2010). Microbial properties of sachet water in India. Journal of Environmental Biology, 31(5), 739-742.
Barnett, H. L., & Hunter, B. B. (1972). Illustrated genera of imperfect fungi (3rd ed.). Burgess Publishing Company.
Bifulco, J. M., Shirey, J. J., & Bissonnette, G. K. (1989). Detection of Acinetobacter spp. in rural drinking water supplies. Applied and Environmental Microbiology, 55(9), 2214–2219.
Buchanan, R. E., & Gibbons, N. E. (Eds.). (1994). Bergey's manual of determinative bacteriology (9th ed.). Williams and Wilkins.
CAWST. (2009). Introduction to drinking water quality testing. Centre for Affordable Water and Sanitation Technology.
Cheesbrough, M. (2010). District laboratory practice in tropical countries, Part 2 (2nd ed.). Cambridge University Press.
Chinenye, N. M., & Amos, D. D. (2017). Microbiological quality of sachet water produced and sold in Awka, Anambra State, Nigeria. International Journal of Scientific and Research Publications, 7(4), 438-442.
Craun, G. F., & McCabe, L. J. (1993). Review of the causes of waterborne-disease outbreaks. Journal of the American Water Works Association, 65(1), 74–84.
Dada, A. C. (2009). Sachet water phenomenon in Nigeria: Assessment of the potential health impacts. African Journal of Microbiology Research, 3(1), 015-021.
DEFRA. (2011). A review of fungi in drinking water and the implications for human health. Department for Environment, Food and Rural Affairs.
Denning, D. W., Kibbler, C. C., & Barnes, R. A. (2006). British Society for Medical Mycology proposed standards of care for patients with invasive fungal infections. The Lancet Infectious Diseases, 6(1), 55–64.
Dodoo, D. K., Quagraine, E. K., & Okai-Sam, F. (2006). Quality of "sachet" waters in the Cape Coast municipality of Ghana. Journal of Environmental Science and Health, Part A, 41(3), 329-342.
Dufour, A., Snozzi, M., & Koster, W. (2002). Assessing microbial safety of drinking water: Improving approaches and methods. World Health Organization.
Edema, M. O., Atayese, A. O., & Bankole, M. O. (2011). Pure water syndrome: Bacteriological quality of sachet-packed drinking water sold in Nigeria. African Journal of Food, Agriculture, Nutrition and Development, 11(2), 4595-4609.
Egurefa, S. O., Okinedo, J. I., Awari, V. G., Ogbonna, U. S. A., Obianom, A. O., Victor-Aduloju, A. T., Abana, C. C., Umeoduagu, N. D., Uwanta, L. I., Agu, K. C., Nwosu, J. C., & Onoyima, L. C. (2024). Assessment of the bacteriological and heavy metal contamination in drinking water from borehole sites in Enugu metropolis. Research Journal1 of Life Science, Bioinformative, Pharmaceuticals and Chemical Science, 10(5), 13-26.
Egwari, L. O., & Aboaba, O. O. (2002). Environmental impact on the bacteriological quality of domestic water supplies in Lagos, Nigeria. African Journal of Clinical and Experimental Microbiology, 3(1), 24-28.
Emenike, P. C., Tenebe, I. T., & Jarvis, P. (2017). Assessment of heavy metal pollution in sachet water sold in Ado-Odo Ota, South-Western Nigeria. Cogent Engineering, 4(1), 1325106.
Emmanuel, E., & Solomon, A. (2012). Quality assessment of sachet and bottled water in the Bolgatanga municipality, Ghana. Research Journal of Applied Sciences, Engineering and Technology, 4(18), 3324-3327.
Eniola, K. I. T., Olayemi, A. B., & Opasina, M. A. (2006). Impact of storage on the quality of water from two sources in Ilorin, Nigeria. Nigerian Journal of Microbiology, 20(1), 1007-1012.
Ezeokoli, C. M., Agu, K. C., Nwosu, J. C., Orji, M. U., Uwanta, L. I., Umeoduagu, N. D., Victor-Aduloju, A. T., & Ikenwa, B. O. (2023). Bacteriological evaluation of Kwata abattoir waste water, Awka, Nigeria. Innovare Journal of Science, 11, 1-4.
Ezeugwunne, I. P., Agbakoba, N. R., & Nnamah, N. K. (2006). The bacteriological quality of sachet water marketed in Nnewi, Nigeria. Journal of Public Health and Epidemiology, 1(1), 001-005.
Falegan, C. R., Akoja, S. O., & Ojo, O. O. (2014). Microbiological quality of sachet water sold in Ado-Ekiti, Nigeria. International Journal of Current Microbiology and Applied Sciences, 3(10), 1020-1030.
Federal Ministry of Health. (n.d.). Health statistics. Federal Republic of Nigeria.
FMWR. (2000). National water supply and sanitation policy. Federal Ministry of Water Resources, Nigeria.
Fong, T. T., Mansfield, L. S., & Wilson, D. L. (2007). Massive microbiological groundwater contamination associated with a waterborne outbreak in Lake Erie. Environmental Health Perspectives, 115(6), 857–864.
Frankova´, E., & Horecka, M. (1995). Filamentous fungi in the drinking water of the city of Bratislava. Folia Microbiologica, 40(2), 201–204.
Frederiksen, H. D. (1996). Water crisis in developing countries: Lessons from the World Bank experience. Natural Resources Forum, 20(3), 203-213.
Gaisie, E., & Gyau-Boakye, P. (2007). Water scarcity in urban areas: The case of Accra, Ghana. Proceedings of the 33rd WEDC International Conference.
Gangil, R., Tripathi, R., Patyal, A., & Dutta, P. (2013). Bacteriological evaluation of packaged bottled water sold in Jaipur city and its public health significance. Veterinary World, 6(1), 27-30.
Garba, Z. N., Hamza, S. A., & Galadima, A. (2008). Arsenic contamination in drinking water in Karaye Local Government Area, Kano State, Nigeria. Journal of Applied Sciences and Environmental Management, 12(2), 39-41.
Garba, Z. N., Sani, A., & Musa, M. A. (2012). Assessment of heavy metals in drinking water sources in Kaduna State, Nigeria. Bayero Journal of Pure and Applied Sciences, 5(1), 61-65.
Gbadegesin, N., & Olorunfemi, F. (2009). Assessment of rural water supply systems in Southwestern Nigeria. African Technology Policy Studies Network.
Georgia, A. (1999). Manual of environmental microbiology. ASM Press.
Hageskal, G., Gaustad, P., & Heier, B. T. (2007). Occurrence of moulds in drinking water. Journal of Applied Microbiology, 102(3), 774–780.
Hageskal, G., Knutsen, A. K., & Gaustad, P. (2009). Diversity and significance of mold species in Norwegian drinking water. Applied and Environmental Microbiology, 75(5), 1588–1597.
Hageskal, G., Lima, N., & Skaar, I. (2006). The study of fungi in drinking water. Mycological Research, 110(Pt 9), 1055–1064.
Hapcioglu, B., Yegenoglu, Y., & Erturan, Z. (2005). Mycological contamination in Turkish domestic kitchens. International Journal of Environmental Health Research, 15(4), 291–298.
Hassan, A. A., El-Kowrany, S. I., & El-Aziz, A. A. (2016). Assessment of the chemical and microbiological quality of bottled and sachet water in Egypt. Journal of Water and Health, 14(2), 324-334.
Ibrahim, A., Uba, A., & Yusuf, I. (2015). Assessment of the quality of sachet and bottled water marketed in Bauchi Metropolis, Nigeria. International Journal of Science and Technology, 4(2), 68-73.
Ilori, O. M., Adewoyin, A. G., & Oyetibo, G. O. (2019). Groundwater quality assessment in a typical urban center in Southwestern Nigeria. Environmental Monitoring and Assessment, 191(2), 1-15.
Isikwue, M. O., & Chikezie, A. (2014). Quality assessment of various sachet water brands marketed in Bauchi metropolis. International Journal of Engineering and Technical Research, 2(5), 1-7.
Jagals, P., Grabow, W. O. K., & Williams, E. (1999). The effect of storage on the microbiological quality of drinking water. Water SA, 25(2), 221-226.
Johnson, T. R., & Case, C. L. (2007). Laboratory experiments in microbiology (8th ed.). Pearson Education.
Justin, I. Z., Odukoya, O. O., & Rowland, L. C. (2012). Quality assessment of sachet water in Lagos State, Nigeria. Journal of Environmental Chemistry and Ecotoxicology, 4(11), 206-214.
Kalpana, S., Sule, H., & Oche, O. (2011). Bacteriological quality of sachet water sold in Kebbi State University and its environs. Science World Journal, 6(2), 1-3.
Kanzler, D., Buzina, W., & Paulitsch, A. (2007). Occurrence and hygienic relevance of fungi in drinking water. Mycoses, 51(2), 165–169.
Kelley, J., Kinsey, G., & Paterson, R. (2003). Identification and control of fungi in distribution systems. AWWA Research Foundation.
Kennedy, D., & Williams, I. (2007). Fungi in potable water distribution systems. Proceedings of the Water Quality Technology Conference.
Kirk, P. M., Cannon, P. F., & David, J. C. (2001). Ainsworth and Bisby's dictionary of the fungi (9th ed.). CABI Publishing.
Klich, M. A. (2002). Identification of common Aspergillus species. Centraalbureau voor Schimmelcultures.
Langfelder, K., Streibel, M., & Jahn, B. (2003). Biosynthesis of fungal melanins and their importance for human pathogenic fungi. Fungal Genetics and Biology, 38(2), 143–158.
Larone, D. H. (2002). Medically important fungi: A guide to identification (4th ed.). ASM Press.
LeChevallier, M. W., & Seidler, R. J. (1980). Staphylococcus aureus in rural drinking water. Applied and Environmental Microbiology, 39(4), 739–742.
Leclere, H., & Moreau, A. (2002). Microbiological safety of natural mineral water. FEMS Microbiology Reviews, 26(2), 207–222.
Li, P., & Qian, H. (2018). Water resource development and protection in loess areas of the world: A summary. Environmental Earth Sciences, 77(13), 1-5.
Li, P., & Wu, J. (2019). Drinking water quality and public health. Exposure and Health, 11(2), 73-79.
Li, P., Wu, J., & Qian, H. (2017). Preliminary assessment of groundwater quality in a shallow aquifer. Exposure and Health, 9(2), 93-104.
Magda, M. H., Ahmed, A. S., & Mohamed, A. G. (2008). Chemical and microbiological quality of bottled water in Egypt. Journal of Applied Sciences Research, 4(6), 697-704.
Mara, D., & Horan, N. (2006). The handbook of water and wastewater microbiology. Academic Press.
Mgbakor, C., Ojiegbu, G. C., & Nnaji, J. C. (2011). Bacteriological quality of sachet water produced and sold in Nnewi, Anambra State, Nigeria. Journal of Research in Environmental Science and Toxicology, 1(8), 195-200.
Michael, J. O., Odey, M. O., & Okon, E. E. (2015). Bacteriological quality of sachet water marketed in Calabar, Nigeria. Global Journal of Pure and Applied Sciences, 21(1), 1-5.
Mintz, E. D., Reiff, F. M., & Tauxe, R. V. (1995). Safe water treatment and storage in the home: A practical new strategy to prevent waterborne disease. JAMA, 273(12), 948–953.
Mirian, I., Copetti, M. V., & Santurio, J. M. (2007). Fungi in drinking water: A potential health hazard. Brazilian Journal of Microbiology, 38(3), 411-415.
Momba, M. N. B., & Kaleni, P. (2002). Regrowth and survival of indicator microorganisms on the surfaces of household containers used for the storage of drinking water in rural communities of South Africa. Water Research, 36(12), 3023–3028.
Momba, M. N. B., Osode, A. N., & Sibewu, M. (2010). The impact of inadequate wastewater treatment on the receiving water bodies. Water Science and Technology, 61(5), 1325-1332.
Montiel, A., Rigal, S., & Welte, B. (1999). Fungal musty taints in drinking water. Water Science and Technology, 40(6), 245–250.
Moreau, C. (1979). Moulds, toxins and food. John Wiley and Sons.
Muaza, M. A., Sule, H., & Enem, S. I. (2012). Microbial quality of sachet water marketed in Maiduguri metropolis, Nigeria. African Journal of Food Science, 6(7), 197-201.
Mufeed, B. (2006). Assessment of bottled water quality in the United Arab Emirates. University of Sharjah.
Muittari, A., Kuusisto, P., & Virtanen, P. (1980). An epidemic of extrinsic allergic alveolitis caused by tap water. Clinical and Experimental Allergy, 10(1), 77–90.
Mukhtar, M. D., & Oyeyi, T. I. (2005). Microbiological and physicochemical assessment of sachet water in Kano, Nigeria. African Journal of Biotechnology, 4(11), 1293-1296.
Musa, M. A., Garba, Z. N., & Sani, A. (2008). Arsenic concentration in drinking water sources in Zaria, Nigeria. Journal of Applied Sciences and Environmental Management, 12(3), 67-69.
Nana, G., & Amorin, R. (2011). Quality of bottled and sachet water in the Tarkwa-Nsuaem municipality of Ghana. Research Journal of Applied Sciences, Engineering and Technology, 3(6), 553-558.
Nataro, J. P., & Kaper, J. B. (1998). Diarrheagenic Escherichia coli. Clinical Microbiology Reviews, 11(1), 142–201.
Ngozi, O. O., Ogbulie, J. N., & Nwabueze, R. N. (2010). Bacteriological quality of sachet water produced and sold in Owerri metropolis, Nigeria. African Journal of Biotechnology, 9(25), 3893-3898.
Nigeria Population Commission. (2006). 2006 Population and housing census of the Federal Republic of Nigeria. National Population Commission.
Nigerian Standard for Drinking Water Quality (NSDWQ). (2007). Standard Organization of Nigeria.
Nikaeen, M., & Mirhendi, H. (2008). Aspergillus contamination in Iranian drinking water. Iranian Journal of Public Health, 37(3), 150-152.
NSDWQ. (2007). Nigerian standard for drinking water quality. Standards Organization of Nigeria.
Nwachukwu, N. C., & Emeruem, N. M. (2007). Antibiotic resistant bacteria in sachet water sold in Umuahia, Nigeria. Journal of Applied Sciences, 7(18), 2639-2643.
Nweze, J. E., Okafor, J. I., & Onyimba, I. A. (2018). Microbiological and physicochemical assessment of sachet water produced in Enugu State, Nigeria. African Journal of Microbiology Research, 12(15), 345-352.
Nwosu, J. N., & Ogueke, C. C. (2004). Evaluation of sachet water quality in Owerri, Nigeria. Journal of Food Technology, 2(2), 67-70.
Nystrom, A., Grimvall, A., & Krantz-Rulcker, C. (1992). Drinking water off-flavour caused by 2,4,6-trichloroanisole. Water Science and Technology, 25(2), 241–249.
Obiri-Danso, K., Okore-Hanson, A., & Jones, K. (2003). The microbiological quality of drinking water sold on the streets of Kumasi, Ghana. Letters in Applied Microbiology, 37(4), 334–339.
Ogundipe, O. (2008). The booming water industry in Nigeria. The Punch Newspaper.
Okafor, E. C., & Ogbonna, D. N. (2003). Physicochemical and microbiological characteristics of water used for fruit juice production in Southeastern Nigeria. Global Journal of Pure and Applied Sciences, 9(4), 525-530.
Okeri, H. A., Akerele, P. O., & Usifoh, C. O. (2009). Trace metal levels in drinking water in Benin City, Nigeria. African Journal of Biotechnology, 8(3), 442-445.
Okonko, I. O., Adejoye, O. D., & Ogunnusi, T. A. (2008). Comparative studies and microbial risk assessment of different water samples used for processing frozen sea foods in Ijora-Olopa, Lagos State, Nigeria. African Journal of Biotechnology, 7(16), 2906-2914.
Okpako, E. C., Osuagwu, A. N., & Duke, E. E. (2009). Fungal contamination of sachet water sold in Calabar, Nigeria. African Journal of Microbiology Research, 3(7), 374-378.
Oladapo, O. T., Ojo, O. T., & Akinwale, O. P. (2009). Bacteriological quality of sachet water sold in Ibadan, Nigeria. African Journal of Biomedical Research, 12(2), 145-148.
Oladipo, I. C., Adejoye, O. D., & Kolawole, O. M. (2009). Bacteriological and physicochemical qualities of sachet water in Ogbomoso, Nigeria. African Journal of Food Science, 3(11), 350-354.
Olayemi, A. B. (2005). Bacteriological quality of water from a water treatment plant and the impact of storage. Global Journal of Pure and Applied Sciences, 11(2), 205-208.
Oluwafemi, F., & Ojo, O. E. (2017). Microbiological quality of sachet water in Lagos State, Nigeria. Journal of Applied Sciences and Environmental Management, 21(2), 245-250.
Omalu, I. C. J., Eze, R. A., & Gbesi, S. (2010). Contamination of sachet water by production and distribution chain in Lagos, Nigeria. African Journal of Food Science, 4(2), 062-066.
Omotayo, S. K., & Denloye, S. A. (2002). The role of NAFDAC in the regulation and control of water products in Nigeria. NAFDAC Publications.
Oni, A. A., & Olayeni, O. A. (2003). Fungal spores in treated pipe-borne water in Ilorin, Nigeria. Journal of Applied Sciences, 6(4), 761-763.
Onifade, A. K., & Ilori, M. O. (2008). Bacteriological quality of sachet water sold in Akure, Nigeria. African Journal of Biomedical Research, 11(2), 245-249.
Onweluzo, J. C., & Akuagbazie, C. A. (2010). Assessment of the quality of sachet water consumed in Onitsha Metropolis, Nigeria. Journal of Food Technology, 8(3), 120-124.
Oparaku, N. F., Mgbenka, B. O., & Ibeto, C. N. (2011). Performance evaluation of a locally fabricated ultraviolet water purification system. International Journal of Physical Sciences, 6(28), 6529-6532.
Oparaocha, E. T., Iroegbu, O. C., & Obi, R. K. (2010). Quality assessment of commercial bottled drinking water. African Journal of Biotechnology, 9(13), 1987-1992.
Oparaocha, E. T., Obunwo, C. C., & Ibe, S. N. (2022). Quality assessment of packaged water in Southeastern Nigeria: A review. International Journal of Environmental Health Research, 32(4), 789-805.
Orisakwe, O. E., Nduka, J. K., & Amadi, C. N. (2006). Heavy metals in sachet water in the Eastern Nigeria. Bulletin of Environmental Contamination and Toxicology, 77(4), 592-598.
Oshibanjo, O. D., Oloruntoba, E. O., & Adekoya, A. E. (2000). Public health analysis of water quality in Lagos, Nigeria. Institute of Public Analysts of Nigeria (IPAN).
Ostoff, S. M. (1994). Yersinia as an emerging pathogen: The role of food and water. Journal of Applied Bacteriology, 76(2), 69S–79S.
Oyagade, J. O., & Fasuan, S. O. (2004). Occurrence of antibiotic resistant strains of Escherichia coli in drinking water sources in Nigerian communities. African Journal of Biotechnology, 3(5), 285-288.
Oyedeji, O., Olutiola, P. O., & Moninuola, M. A. (2010). Microbiological quality of packaged drinking water brands marketed in Ibadan metropolis and Ile-Ife town in Southwestern Nigeria. African Journal of Microbiology Research, 4(1), 096-102.
Oyeku, O. M., & Omotayo, F. O. (2001). Water quality assessment of some selected boreholes in Lagos State, Nigeria. Proceedings of the International Conference on Water and Environment.
Oyeku, O. M., Omotayo, F. O., & Odukoya, O. O. (2001). Water quality assessment of some selected boreholes in Lagos State, Nigeria. Proceedings of the International Conference on Water and Environment.
Oyelude, E. O., & Ahenkorah, S. (2012). Quality of sachet water and bottled water in Bolgatanga municipality of Ghana. Research Journal of Applied Sciences, Engineering and Technology, 4(18), 3324-3327.
Oyetunji, T. P. (2016). Diagram of ultra violet filtration for small scale sachet water production.
Paterson, R. R. M. (2006). Fungi and fungal toxins as weapons. Mycological Research, 110(Pt 9), 1003–1010.
Payment, P., Waite, M., & Dufour, A. (1997). Introducing parameters for the assessment of drinking water quality. World Health Organization.
Pierre, L. (1999). Water quality and health. World Health Organization.
Pitt, J. I., & Hocking, A. D. (1999). Fungi and food spoilage (2nd ed.). Aspen Publishers.
Popoola, O. O., Ojo, D. A., & Adejumobi, D. A. (2007). Microbial quality of stored household water in plastic containers in Ibadan, Nigeria. African Journal of Clinical and Experimental Microbiology, 8(3), 106-114.
Priyanka, S., Verma, S. K., & Khan, A. (2010). Water quality assessment of groundwater in and around Perumbakkam area, Kancheepuram district, Tamil Nadu. Journal of Industrial Pollution Control, 26(1), 25-30.
Rajiv, P., Hasna, A. S., & Kamaraj, M. (2012). Physicochemical and microbial analysis of different water samples used for domestic purposes. International Journal of Environmental Sciences, 2(3), 1462-1466.
Rosenberg, F. A. (2003). The effect of storage on the pH of drinking water. Unpublished manuscript.
Salle, A. J. (1973). Fundamental principles of bacteriology (7th ed.). McGraw-Hill.
Samson, R. A., Hoekstra, E. S., Frisvad, J. C., & Filtenborg, O. (Eds.). (2004). Introduction to food- and airborne fungi. Centraalbureau voor Schimmelcultures.
Sharma, S., Chhipa, R. C., & Kori, M. L. (2011). Analysis of physicochemical parameters of water from various sources. International Journal of Pharmaceutical Sciences and Research, 2(6), 1540-1546.
Shelton, T. B., Hocking, M. B., & Klapecki, S. (2005). Lead in drinking water: A technical review. Environmental Protection Agency.
Stewart, J. R., Gast, R. J., & Fujioka, R. S. (2007). The coastal environment and human health: Microbial indicators, pathogens, sentinels and reservoirs. Environmental Health, 7(Suppl 2), S3.
Sunday, J. T., Akinde, S. B., & Obire, O. (2011). Effect of storage on the physicochemical and bacteriological quality of sachet water in Port Harcourt, Nigeria. Nature and Science, 9(11), 1-5.
Swerdlow, D. L., Woodruff, B. A., & Brady, R. C. (1997). A waterborne outbreak in Missouri of Escherichia coli O157:H7 associated with bloody diarrhea and death. Annals of Internal Medicine, 117(10), 812–819.
Taiwo, A. G., Adeigbe, O. O., & Ogunbiyi, T. A. (2012). Bacteriological quality of sachet water in Abeokuta, Nigeria. Journal of Environmental Science and Water Resources, 1(2), 6-10.
Tambekar, D. H., Hirulkar, N. B., & Kalikar, M. V. (2006). Microbial quality of drinking water in storage containers. Journal of Environmental Biology, 27(2), 349-350.
Tanveer, A., Hasan, M. U., & Ali, A. (2011). Environmental factors affecting fungal growth in water. Pakistan Journal of Botany, 43(6), 2977-2983.
Thliza, I. A., Yahaya, A., & Doko, M. H. I. (2015). Assessment of heavy metals in sachet water sold in Samaru, Zaria, Nigeria. Journal of Applied Sciences and Environmental Management, 19(1), 139-143.
Uddin, M. G., Nash, S., & Olbert, A. I. (2021). A review of water quality index models and their use for assessing surface water quality. Ecological Indicators, 122, 107218.
USAID. (1990). Nigeria water and sanitation sector assessment. United States Agency for International Development.
USEPA. (2006). National primary drinking water regulations. United States Environmental Protection Agency.
USEPA. (2012). 2012 Edition of the Drinking Water Standards and Health Advisories. United States Environmental Protection Agency.
Vachee, A., Mossel, D. A. A., & Leclerc, H. (1997). A study of the fate of the autochthonous bacterial flora of still mineral waters. Journal of Applied Microbiology, 82(3), 299–307.
Victor-Aduoju, A. T., Okonkwo, N. N., Okoli, F. A., Agu, K. C., Okoye, C. W., Awari, V. G., & Umeoduagu, N. D. (2023). Comparative analysis of microbial load of water in selected hostels in Ifite, Awka. International Journal of Progressive Research in Engineering Management and Science, 3(9), 400-408.
Waage, A. S. (1999). Detection and characterization of Yersinia enterocolitica in drinking water. Norwegian School of Veterinary Science.
Waipara, N. W. (1998). The effect of ultraviolet radiation on fungal spores in water. University of Auckland.
Warburton, D. W., Bowen, B., & Konkle, A. (1992). The survival and recovery of Pseudomonas aeruginosa and its effect upon the bacterial flora of bottled natural mineral water. Journal of Food Protection, 55(11), 888–892.
Watanabe, T. (2002). Pictorial atlas of soil and seed fungi: Morphologies of cultured fungi and key to species (2nd ed.). CRC Press.
WHO. (1984). Guidelines for drinking-water quality: Volume 2—Health criteria and other supporting information. World Health Organization.
WHO. (1993). Guidelines for drinking-water quality: Volume 1—Recommendations (2nd ed.). World Health Organization.
WHO. (1997). Guidelines for drinking-water quality: Volume 3—Surveillance and control of community supplies (2nd ed.). World Health Organization.
WHO. (2008). Guidelines for drinking-water quality: Volume 1—Recommendations (3rd ed.). World Health Organization.
WHO. (2010). Water for health: WHO guidelines for drinking-water quality. World Health Organization.
WHO. (2011). Guidelines for drinking-water quality (4th ed.). World Health Organization.
Wu, J., Li, P., & Qian, H. (2019). Hydrochemical characterization of drinking groundwater with special reference to fluoride in an arid area of China. Exposure and Health, 11(2), 81-92.
Yahaya, A., Adegoke, A. A., & Ibrahim, Y. K. E. (2011). Bacteriological quality of drinking water from source to point of use in Zaria, Nigeria. African Journal of Microbiology Research, 5(23), 3907-3916.
Zakaria, I. (2012). NAFDAC and the regulation of sachet water in Nigeria. The Guardian Newspaper.
Downloads
Published
How to Cite
Issue
Section
Categories
License
Copyright (c) 2026 VC Ifionu, Kingsley Agu, P.AN Oghonim, L.I Uwanta, VG Awari, C.A Anazodo, OC Soludo, I.K. Ojeah, IAC Mbachu, J.I Okinedo

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-ShareAlike that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).














